Thick films - parasites and debris: Difference between revisions
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File:12 Mess & troph.jpg|<span style="font-size:80%">'''A'''</span>|link={{filepath: | File:12 Mess & troph.jpg|<span style="font-size:80%">'''A'''</span>|link={{filepath:12 Mess & troph.jpg}} | ||
File:12 Mess & troph anno.jpg|<span style="font-size:80%">'''B'''</span>|link={{filepath:12 Mess & troph anno}} | File:12 Mess & troph anno.jpg|<span style="font-size:80%">'''B'''</span>|link={{filepath:12 Mess & troph anno}} | ||
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Revision as of 11:45, 21 February 2025
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>>>Current page: Distinguishing features on thick film
"Debris"
The parasites on a thick film lie within a background of white cells, platelets and various red cell components that have not been fully lysed. It is important to recognise these different features (which may vary a little between films depending on thickness and staining). A film area without parasites is shown below.
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A
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B
Normal background elements of a thick malaria film. The neutrophil (A) is recognisable by the characteristic nuclear shape but show artefactual distortion: in this case the chromatin detail is lost and there is no visible membrane outline or granular content. A careful look at the group of structures (B) suggests that they are part of a large group of clumped platelets formed when the film was prepared. Finally, the more amorphous material (C) most likely represents fibrin clot and un-lysed red cell debris.
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A
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B
Subtle single parasite
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A
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B
Two parasites with debris
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A
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B
3 parasites