Thick vs Thin film comparison

---

Navigation
>Main Malaria Index
>>Thick film - main page
>>>Current page: Thick film vs thin film

---

Relative merits of thick or thin films in malaria diagnosis

---

• 

  Thin film appearances P.falciparum

• 

  Thick film appearances P.falciparum

Feature	Thick Film	Thin Film
Sensitivity for detection	Higher: detects low parasitaemia ~5–10 parasites/µL	Lower: generally needs ~50 parasites/µL for reliable detection)
Species Identification	Poor: RBC morphology lost and species-specific features may be difficult	Excellent: Parasite morphology and RBC characteristics are readily observed
Quantification of parasitaemia	Difficult: requires estimation so is imprecise	Easier: parasites can be counted per number of RBCs
Preparation and staining	Longer: requires air drying before careful staining to avoid artefact	Faster: films are fixed and stained immediately with clearer morphology

TABLE: A COMPARISON OF THICK AND THIN FILMS

1. Sensitivity for detection
Thick film: Higher - can detect lower levels of parasitaemia ~5–10 parasites/µL
Thin film: Lower - generally requires ~50 parasites/µL for reliable detection
2. Species Identification
Poor: poor - red cell morphology is lost and species-specific features may not be seen
Thin film: Good - both parasite morphology and RBC characteristics are assessed
3. Quantification of parasite number
Difficult: red cells are not seen so requires estimation and is therefore imprecise
Easier: parasites can be counted counted directly and assessed as a proportion of red cells
4. Preparation and staining considerations
Less easy: requires air drying and careful spreading and staining to minimise any artefact
Easier: films are fixed and stained immediately with clearer morphology of parasites and red cells

---