Staining pH description: Difference between revisions

Latest revision as of 21:39, 4 January 2025

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Effect of stain pH on parasite appearance The best detection and species identification in malaria is made when staining is performed at a slightly more alkaline pH than is usually employed (pH 7.4). This pH makes parasites more visible since the surrounding red cells become grey, and structures such as cytoplasmic dots are made more visible. Although parasites and additional features are detectable using standard staining pH allowing both parasite-detection and species-identification both processes are more readily performed at pH7.4. A B C Trophozoites of P.vivax stained at pH7.4 (A) or pH6.9 (B ad C). Note that staining at alkaline (pH 7.4) gives the red cell a pale blue/grey colour when compared to the acidic conditions (pH 6.9), with these conditions making both the trophozoite and the added cytoplasmic dots more visible. Where pH7.4 is used the key features of parasite shape and red cell morphology can still be seen, but parasites may be less evident and dots fainter (B) or absent (C).

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 |colspan="1" style = "font-size:140%; color:black; background: FFFAFA"|<span style="color:black>'''Effect of stain pH on parasite appearance'''</span>
+<span style="font-size:90%"></br></br>The best detection and species identification in malaria is made when staining is performed at a slightly more alkaline pH than is usually employed (pH 7.4). This pH makes parasites more visible since the surrounding red cells become grey, and structures such as cytoplasmic dots are made more visible. Although parasites and additional features are detectable using standard staining pH allowing both parasite-detection and species-identification both processes are more readily performed at pH7.4.</span>
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-Trophozoites of ''P.vivax'' stained at pH7.4 (A) or pH6.9 (B ad C). Note that staining at the more alkaline conditions (pH7.4) gives the red cell a pale blue colour when compared to the acidic conditions (pH6.9), with these conditions making both the trophozoite and the added cytoplasmic dots more visible. Where pH7.4 is used the key features of parasite shape and red cell morphology can still be seen, but parasites are less evident and dots may be fainter (B) or absent (C).
+<span style="font-size:90%">''Trophozoites of P.vivax stained at pH7.4 (A) or pH6.9 (B ad C). Note that staining at alkaline (pH 7.4) gives the red cell a pale blue/grey colour when compared to the acidic conditions (pH 6.9), with these conditions making both the trophozoite and the added cytoplasmic dots more visible. Where pH7.4 is used the key features of parasite shape and red cell morphology can still be seen, but parasites may be less evident and dots fainter (B) or absent (C).''
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-<span style="color:navy>'''Description'''</span>
-The best detection and species identification in malaria is made when staining is performed at a slightly more alkaline pH - this makes parasites more visible compared with the grey of the surrounding red cells and makes some structures such as cytoplasmic dots more visible. These features are detectable at standard staining pH and both detection and species identification can be performed, it is just less easy.

Staining pH description: Difference between revisions

From MalariaETC

Latest revision as of 21:39, 4 January 2025