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RDT test: formats of tests

From MalariaETC

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TEST FORMATS


Single test-band RDT tests


This test format usually uses the HRP2 antigen to detect P.falciparum and offers particular advantages:

  • simplicity of interpretation
  • lower production cost
  • stability for storage and transport
  • valuable where a single malaria species dominates.


Use: The single species test for P.falciparum is very useful in Africa areas where infections with this species account for more than 95% of infections and may be used where technical expertise is less available. However, this is at the cost missing less prevelant species, and the lack of a pan-malaria antibody means that cases with HRP2-gene delection may be missed.



Multi-band RDT tests


These use two (or occasionally more) test bands together with a control band.

Most often one band is a species-specific antibody that identifies the dominant (or most important) species for the selected region. The second band generally employs a pan-malaria antibody that may' detect other species. The most frequent format is P.falciparum-specific combined with a Pan-malaria-specific antibdy.

This format can be very effective providing its' limitations are understood by users. In particular the poor sensitivity of pan-malarial antibodies for some species.