RDT test: design and function: Difference between revisions
From MalariaETC
(Created page with "'''The basic principle is:'''</br> If a patient has a malaria infection:>/br> (1) Dye-labelled antibodies will bind to specific parasite proteins forming an antigen-antibody complexes.</br> (2) These complexes are then captured by a second antibody immoblised on a nitro-cellulase forming a visible band.</br> Precise test formats vary but have common principles - this is illustrated for a single parasite antigen below (although many tests will test for multiple antigen...") |
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'''The basic principle is:'''</br> | '''The basic principle is:'''</br> | ||
If a patient has a malaria infection: | If a patient has a malaria infection:</br> | ||
(1) | (1) A blood sample will be lysed in buffer to release parasite antigens, and expose them to dye-labelled antibodies</br> | ||
(2) These complexes are then captured by a second antibody immoblised on a nitro-cellulase | (2) The labelled antibodies will bind to parasite proteins forming an antigen-antibody complexes.</br> | ||
(2) These complexes are then captured by a second antibody immoblised on a nitro-cellulase to form a visible band.</br> | |||
Test formats vary, but share common principles - this is illustrated for a single parasite antigen below.</br> | |||
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Revision as of 11:35, 25 June 2024
The basic principle is:
If a patient has a malaria infection:
(1) A blood sample will be lysed in buffer to release parasite antigens, and expose them to dye-labelled antibodies
(2) The labelled antibodies will bind to parasite proteins forming an antigen-antibody complexes.
(2) These complexes are then captured by a second antibody immoblised on a nitro-cellulase to form a visible band.
Test formats vary, but share common principles - this is illustrated for a single parasite antigen below.
(1) In a typical test the blood sample is loaded into one window, and a lysis buffer in a second window. The lysed blood and parasite antigens then meet a parasite-specific antibody labelled with dye (show strip and mixing of agents = A). (2) Where the parasite-antigen is present, the dye-labelled antibody will bind to that antigen to form a complex (binding=B). This dye-labelled antigen/antibody complex then diffuses along the strip until it encounters a second parasite-specific antibody immobilised as a band on the strip. This immobilised antibody “captures” the labelled antibody/antigen complex to form a visible line (capture 1 = C). (3) The remaining lysed sample (containing labelled antibody not bound to parasite antigen) continues to diffuse along the strip. And encounters a further immobilised antibody that captures it. This forms a control line which indicates that test has been successfully performed (capture 2 = D).