RDT test: formats of tests: Difference between revisions
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<span style="font-size:90%">This test format usually uses the HRP2 antigen to detect ''P.falciparum'' | <span style="font-size:90%">This test format usually uses the HRP2 antigen (or Ff-LDH) to detect infection by ''P.falciparum''. The format offers particular advantages:</span> | ||
*<span style="font-size:90%"> | *<span style="font-size:90%">Simple interpretation | ||
*<span style="font-size:90%">lower production cost | *<span style="font-size:90%">lower production cost | ||
*<span style="font-size:90%"> | *<span style="font-size:90%">Stability (of HRP2) for storage and transport | ||
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<span style="font-size:90%">'''Use:''' The single band test detecting only ''P.falciparum'' is very useful in Africa, since in many areas infections with this species account for more than 95% of malarial infection. The test is very useful in more remote areas where technical expertise in microscopy and interprtation is often less available. However, this is at the cost missing less prevelant species, and where [ [[HRP2-gene deletion]] ] is frequent infection may be missed. | |||
<span style="font-size:90%">'''Use:''' The single | |||
Revision as of 15:43, 24 September 2024
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| TEST FORMATS |
Single test-band RDT tests
This test format usually uses the HRP2 antigen (or Ff-LDH) to detect infection by P.falciparum. The format offers particular advantages:
- Simple interpretation
- lower production cost
- Stability (of HRP2) for storage and transport
Use: The single band test detecting only P.falciparum is very useful in Africa, since in many areas infections with this species account for more than 95% of malarial infection. The test is very useful in more remote areas where technical expertise in microscopy and interprtation is often less available. However, this is at the cost missing less prevelant species, and where [ HRP2-gene deletion ] is frequent infection may be missed.
Multi-band RDT tests
These use two (or occasionally more) test bands together with a control band.
Most often one band is a species-specific antibody that identifies the dominant (or most important) species for the selected region. The second band generally employs a pan-malaria antibody that may' detect other species. The most frequent format is P.falciparum-specific combined with a Pan-malaria-specific antibdy.
This format can be very effective providing its' limitations are understood by users. In particular the poor sensitivity of pan-malarial antibodies for some species.