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Practical reasons for poor RDT performance: Difference between revisions

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{| class="wikitable" style="border-style: solid; border-width: 4px; color:black"
{| class="wikitable" style="widthe:90%; border-style: solid; border-width: 4px; color:black"
|colspan="1" style = "font-size:100%; color:black; background: FFFAFA"|<span style="color:navy>'''The value of RDT compared with microscopy: thick blood films'''</span>
|colspan="1" style = "font-size:100%; color:blasck; background: FFFAFA"|<span style="color:navy>'''Practical considerations '''</span>


<span style="font-size:90%">Bibilography: [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652944/], [https://www.who.int/teams/global-malaria-programme/case-management/diagnosis/rapid-diagnostic-tests]</span>




Assay quality: not all are the same WHO
<span style="font-size:90%">'''1. Selection and procurement of MDTs'''</br>
Heat stability of the RDT card: storage
 
Age of card or reagent:storage
<span style="font-size:90%">(a) Quality of test design/production. There may be significant variation of performance between tests, users are advised to check test performance (see WHO testing information ref 2 above)</br>(b) Lot variability. Variability between different production batches has been observed and should be considered if RDT performance is less than expected.</br>
Lot to lot variability in assay quality
 
Operator-Specific Factors
<span style="font-size:90%">'''2. Stability and storage issues'''</br>
Operator-Inappropriate placement of reagent or blood drop
 
Operator-Interpreting faint line
<span style="font-size:90%">(a) RDTs typically have a shelf-life of 18–24 months providing storage is appropriateR.</br>(b) Tests can suffer degradation if stored in inappropriate heat or humidity: generally tests are stable in the range 2–30°C or higher, but these limits may be exceeded in many countries, and continuous product refrigeration to the point of use may not be guarenteed in developing countries.
 
<span style="font-size:90%">'''3. Operator training'''</br>
 
<span style="font-size:90%">(a)Problems in test usage may include inappropriate placement of test reagents or blood sample on RDT.</br>(b)Poor interpretion of faint lines.</br>(c) Inadequate training or reference materials

Latest revision as of 16:05, 6 October 2024


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>>>Current page: Practical reasons for test failure



Practical considerations

Bibilography: [1], [2]


1. Selection and procurement of MDTs

(a) Quality of test design/production. There may be significant variation of performance between tests, users are advised to check test performance (see WHO testing information ref 2 above)
(b) Lot variability. Variability between different production batches has been observed and should be considered if RDT performance is less than expected.

2. Stability and storage issues

(a) RDTs typically have a shelf-life of 18–24 months providing storage is appropriateR.
(b) Tests can suffer degradation if stored in inappropriate heat or humidity: generally tests are stable in the range 2–30°C or higher, but these limits may be exceeded in many countries, and continuous product refrigeration to the point of use may not be guarenteed in developing countries.

3. Operator training

(a)Problems in test usage may include inappropriate placement of test reagents or blood sample on RDT.
(b)Poor interpretion of faint lines.
(c) Inadequate training or reference materials